NCAM2 (extracellular) Blocking Peptide (#BLP-NR042) is the original antigen used for immunization during Anti-NCAM2 (extracellular) Antibody (#ANR-042) generation. The blocking peptide binds and ‘blocks’ Anti-NCAM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human LNCaP prostate carcinoma (lanes 5 and 7) and MCF-7 breast adenocarcinoma (lanes 6 and 8) cell lysates:
- Expression of NCAM2 in mouse brainImmunohistochemical staining of mouse free-floating frozen brain sections using Anti-NCAM2 (extracellular) Antibody (#ANR-042). A. In the hippocampal CA3 region, NCAM2 immunoreactivity (green) appears in the pyramidal layer (P, arrows). B. In the olfactory bulb, NCAM2 staining (green) appears in the Mitral cell layer (MCL, arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
- (C)KEDQWLEKKVQGNKDH, corresponding to amino acid residues 635-650 of mouse NCAM-2 (Accession O35136).