NETO1 (extracellular) Blocking Peptide (#BLP-GC045) is the original antigen used for immunization during Anti-NETO1 (extracellular) Antibody (#AGC-045) generation. The blocking peptide binds and ‘blocks’ Anti-NETO1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Expression of NETO1 in rat and mouse cortex.Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-NETO1 (extracellular) Antibody (#AGC-045), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NETO1 staining in rat (A) and mouse (B) sections (green), is detected in cells in the deep pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
- (C)KKGTEKQITPETQK, corresponding to amino acid residues 24-37 of mouse NETO1 (Accession Q8R4I7).