Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Neuronal Pentraxin Receptor (NPTXR) (extracellular) Blocking Peptide (#BLP-NR193) is the original antigen used for immunization during Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193) generation. The blocking peptide binds and ‘blocks’ Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193), (1:400).
3-4. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody, preincubated with Neuronal Pentraxin Receptor (NPTXR)(extracellular) Blocking Peptide (BLP-NR193).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):1-2. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193), (1:400).
3-4. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody, preincubated with Neuronal Pentraxin Receptor (NPTXR)(extracellular) Blocking Peptide (BLP-NR193).
Expression of Neuronal Pentraxin Receptor in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193), (1:1200), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. Staining in the rat hippocampal CA3 region, showed NPTXR immunoreactivity (red) in neuronal profiles (arrows) in the pyramidal layer (P). B. Pre-incubation of the antibody with Neuronal Pentraxin Receptor (NPTXR)(extracellular) Blocking Peptide (BLP-NR193), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RGLQDAGPRRDTMAD, corresponding to amino acid residues 162 - 176 of mouse NPTXR (Accession Q99J85).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
