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Certificate of Analysis

Neuropeptide Y Receptor Antibody Explorer Kit

A Screening Package of Neuropeptide Y Receptor Antibodies Economically Priced

Cat #: AK-540
Size: 4 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-NPY1R Antibody Cat #: ANR-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, R
Immunogen
Peptide (C)RLKRRNNMMDKMRDNK, corresponding to amino acid residues 237-252 of human NPY1R (Accession P25929). 3rd intracellular loop.
Homology
Rat, mouse - 14/16 amino acid residues identical.

Anti-NPY2R Antibody Cat #: ANR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide CEQRLDAIHSEVSMTFKAK, corresponding to amino acid residues 346-364 of mouse NPY2R (Accession P97295). Intracellular, C-terminus.
Homology
Rat - identical; human - 18/19 amino acid residues identical.

Anti-NPY4R Antibody Cat #: ANR-024

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide NINFKKDIKALVLTC corresponding to amino acid residues 326-340 of rat NPY4R (Accession Q63447). Intracellular, C-terminus.
Homology
Mouse - identical; human - 13/15 amino acid residues identical.

Anti-NPY5R Antibody Cat #: ANR-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R (Accession Q63634). 3rd intracellular loop.
Homology
Mouse, bovine, canis - identical; human - 15/16 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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