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Certificate of Analysis

Neurotrophin Receptor Antibody Explorer Kit

A Screening Package of Neurotrophin Receptor Antibodies Economically Priced

Cat #: AK-238
Size: 6 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-p75 NGF Receptor (intracellular) Antibody Cat #: ANT-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CKQNKQGANSRPVNQT corresponding to amino acid residues 278-293 of human p75NTR (Accession P08138). Intracellular, juxtamembrane domain.
Homology
Mouse, rat - identical.

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.

Anti-Sortilin (extracellular) Antibody Cat #: ANT-009

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin (Accession Q99523). Extracellular domain.
Homology
Mouse, rat - identical.

Anti-TrkA (extracellular) Antibody Cat #: ANT-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739). 2nd extracellular immunoglobulin-like domain.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Anti-TrkB (extracellular) Antibody Cat #: ANT-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 of rat TrkB (Accession Q63604). Extracellular domain.
Homology
Mouse, human - 13/14 amino acid residues identical.

Anti-TrkC (extracellular) Antibody Cat #: ANT-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)GHFLKEPFPESTD, corresponding to amino acid residues 393-405 of rat TrkC (Accession Q03351). Extracellular, N-terminus.
Homology
Mouse, human - identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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