NGL-3/LRRC4B (extracellular) Blocking Peptide (#BLP-NR163) is the original antigen used for immunization during Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163) generation. The blocking peptide binds and ‘blocks’ Anti-NGL-3/LRRC4B (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat new born brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Western blot analysis of mouse brain lysate:
- Expression of NGL-3 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-3/LRRC4B (extracellular) Antibody (#ANR-163), (1:200), followed by goat anti-rabbit-AlexaFluor-488. NGL-3 immunoreactivity (green) is detected in the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
- (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3 (Accession P0C192).