Name: Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide
Brand: Alomone Labs
SKU: BLP-NC002

Overview

Cat #: BLP-NC002

Type: Synthetic peptide

Form: Lyophilized powder

Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Applications: wb, ihc

For research purposes only, not for human use

Applications

Demonstration of Pre-adsorption control

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
1,2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:400).
3,4. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
1. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:200).
2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
Expression of nAChRα2 in rat deep cerebellar nucleus
Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChRα2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).

Properties

Sequence

(C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRα2 (Accession P12389).

Accession (Uniprot) Number P12389

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

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Shipping and Ordering information

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Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat nAChRα2. Anti-Nicotinic Acetylcholine Receptor α2 (extracellular) (CHRNA2) Antibody (#ANC-002) can be used in western blot, immunohistochemistry and immunocytochemistry applications. The antibody is specially suited to recognize nAChRα2 in live cells. It has been designed to recognize nAChRα2 from rat mouse and human samples.

Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002)

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Order Summary

Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide

Overview

Application key:

Applications

Properties

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