Overview
Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Application key:
Applications
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:400).
3,4. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002). - Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:200).
2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002). - Expression of nAChRα2 in rat deep cerebellar nucleusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChRα2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).
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