Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:400).
3,4. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:1. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:200).
2. Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
Expression of nAChRα2 in rat deep cerebellar nucleusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChRα2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRα2 (Accession P12389).
Accession (Uniprot) Number P12389
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
