Overview
Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) Blocking Peptide (#BLP-NC006) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006), (1:1000).
3,4. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) Blocking Peptide (#BLP-NC006).
Expression of nAChRα6 in rat striatumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006), (1:100). A. nAChRα6 staining (red) is detected in the striatal matrix (M) but not in the striatal patches (arrows) nor in the overlying corpus callosum (CC). B. DAPI (blue) counterstain reveals the outline of the striatal matrix and patches and the overlying corpus callosum.
Western blot analysis of rat brain membrane lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006-GP), (1:200).
3-4. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Blocking Peptide (BLP-NC006).
Properties
- (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRα6 (Accession P43143).
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