Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) Blocking Peptide (#BLP-NC006) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006), (1:1000).
3,4. Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α6/CHRNA6 (extracellular) Blocking Peptide (#BLP-NC006).
Expression of nAChRα6 in rat striatumImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006), (1:100). A. nAChRα6 staining (red) is detected in the striatal matrix (M) but not in the striatal patches (arrows) nor in the overlying corpus callosum (CC). B. DAPI (blue) counterstain reveals the outline of the striatal matrix and patches and the overlying corpus callosum.
Western blot analysis of rat brain membrane lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody (#ANC-006-GP), (1:200).
3-4. Guinea Pig Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Blocking Peptide (BLP-NC006).
Properties
Sequence
- (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRα6 (Accession P43143).
Accession (Uniprot) Number P43143
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
