Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide (#BLP-NC011) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (#ANC-011) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart lysates:1,2. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (#ANC-011), (1:500).
3,4. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide (#BLP-NC011).
Properties
Sequence
- (C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRβ1 (Accession P25109).
Accession (Uniprot) Number P25109
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
