Overview
Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide (#BLP-NC011) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (#ANC-011) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) heart lysates:1,2. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody (#ANC-011), (1:500).
3,4. Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide (#BLP-NC011).
Properties
- (C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRβ1 (Accession P25109).
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