Overview
Type: Synthetic peptide
Form: Lyophilized powder
Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) Blocking Peptide (#BLP-NC012) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4) and rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysate:1-3. Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012), (1:200).
4-6. Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) Blocking Peptide (#BLP-NC012).- Mouse pancreas sections (1:200) (Ganic, E. et al. (2016) Cell Rep. 14, 1991.).
Properties
Sequence
- (C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChRβ2 (Accession P12390).
Accession (Uniprot) Number P12390
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
