Overview
Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) Blocking Peptide (#BLP-NC012) is the original antigen used for immunization during Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012) generation. The blocking peptide binds and ‘blocks’ Anti-Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications
Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4) and rat (lanes 2 and 5) and mouse (lanes 3 and 6) brain lysate:1-3. Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody (#ANC-012), (1:200).
4-6. Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor β2/CHRNB2 (extracellular) Blocking Peptide (#BLP-NC012).- Mouse pancreas sections (1:200) (Ganic, E. et al. (2016) Cell Rep. 14, 1991.).
Properties
- (C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChRβ2 (Accession P12390).
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