Overview
Type: Synthetic peptide
Form: Lyophilized powder
NMDAR1/GluN1 (extracellular) Blocking Peptide (#BLP-GC001) is the original antigen used for immunization during Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR1/GluN1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:600).
2. Anti-NMDAR1 (GluN1) (extracellular) Antibody, preincubated with NMDAR1/GluN1 (extracellular) Blocking Peptide (#BLP-GC001).
Multiplex staining of NMDAR1 and CALHM1 in mouse hippocampal CA1 regionImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:200) and Anti-CALHM1-ATTO Fluor-594 Antibody (#ACC-101-AR), (1:60). A. Sections were stained with Anti-NMDAR1 (GluN1) (extracellular) Antibody, followed by goat-anti-rabbit-Cy2 (green). Staining reveals expression in neurons of the pyramidal layer (an arrow points at the layer). B. The same section was incubated with Anti-CALHM1-ATTO Fluor-594 Antibody (red). C. Merge of the two images demonstrates colocalization of NMDAR1 and CALHM1 in pyramidal neurons.
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1.2. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:200).
3.4. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody, preincubated with NMDAR1/GluN1 (extracellular) Blocking Peptide (#BLP-GC001).
Multiplex staining of NMDAR1 and M4 muscarinic acetylcholine receptor in mouse parietal cortexImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using rabbit Anti-CHRM4 Antibody (#AMR-004), (1:400) and Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:1200). A. M4 muscarinic receptor staining (green). B. NMDAR1 staining (red) in same section. C. Merge of the two images reveals several cells expressing both M4 muscarinic receptor and NMDAR1 (arrows). Cell nuclei were stained with DAPI (blue).
Multiplex staining of NMDAR1 and ASCT1 in mouse hippocampusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:600) and rabbit Anti-ASCT1 (SLC1A4) Antibody (#ANT-081), (1:300). A. NMDAR1 (in red) appears in profiles of hippocampal dentate gyrus interneurons (arrows). B. ASCT1 (in green) appears in the extracelullar matrix of the subgranular layer (SGL). C. Merge of the two images demonstrates adjacent localization in several neurons (arrows). Cell nuclei are stained with DAPI (blue). GL = granule layer.
Multiplex staining of NMDA3B and NMDAR1 in mouse cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NMDAR3B (GRIN3B) (extracellular) Antibody (#AGC-031), (1:400), followed goat anti-rabbit-AlexaFluor-488 and Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:1200), followed by goat anti-donkey-biotin and streptavidin-Cy3. A. NMDA3A immunoreactivity (green) appears in pyramidal cells of layer 5. B. NMDAR1 immunoreactivity (red) appears in several cell types in the same region. C. Merge of the two images reveals several cells expressing both NMDA3B and NMDAR1 (arrows point at examples). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat NMDA receptor 1 (Accession P35439).
Accession (Uniprot) Number P35439
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
