Overview
Type: Synthetic peptide
Form: Lyophilized powder
NMDAR2A/GluN2A (extracellular) Blocking Peptide (#BLP-GC002) is the original antigen used for immunization during Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002) generation. The blocking peptide binds and ‘blocks’ Anti-NMDAR2A/GluN2A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates:1. Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:600).
2. Anti-NMDAR2A (GluN2A) (extracellular) Antibody, preincubated with NMDAR2A/GluN2A (extracellular) Blocking Peptide (#BLP-GC002).
Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60) and Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDAR2A (GluN2A) (extracellular) Antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus. Arrows point at an example of NR2A and NR2B co-expression.
Expression of NR2A in rat hippocampusImmunohistochemical staining of rat hippocampal dentate gyrus with Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002). A. NMDAR2A (green) appears diffusely in the outer molecular layer of the dentate gyrus (Out Mol.) and in cells along the subgranular layer (arrows). B. Staining of parvalbumin (PV, red) identifies interneurons in the dentate gyrus. C. Confocal merge demonstrates localization of PV in some neurons with NMDAR2A.
Properties
Sequence
- GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A (Accession Q00959).
Accession (Uniprot) Number Q00959
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
