NNC 55-0396 dihydrochloride

A Blocker of T-type CaV Channels
Cat #: N-205
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 564.57
    Purity: >95%
    Form Lyophilized powder.
    Effective concentration 1-100 µM.
    Chemical name (1S,2S)-2-[2-[[3-(1H-Benzimidazol-2­-yl)propyl]methylamino]ethyl]-6-fluoro-1,2,3,4-tet­rahydro-1-(1-methylethyl)-2-naphthalenyl cyclopropanecarboxylate dihydrochloride.
    Molecular formula C30H39.8Cl1.8FN3O2.
    CAS No.: 357400-13-6.
    Activity NNC 55-0396 displays IC50 values of 6.8 and >100 μM for inhibition of CaV3.1 T-type channels and HVA currents respectively in INS-1 cells1.
      • Huang, L. et al. (2004) J. Pharmacol. Exp. Ther309, 193.

    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility 50 mM in water. Centrifuge all product preparations before use (10000 x g for 1 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
      • Alomone Labs NNC 55-0396 dihydrochloride inhibits CaV3.2 channels heterologously expressed in Xenopus oocytes.
        A. Time course of CaV3.2 peak current amplitude, elicited by 100 ms voltage step from holding potential of -100 mV to -20 mV, delivered every 10 seconds. Application of 10 µM NNC 55-0396 dihydrochloride (#N-205) inhibits the CaV3.2 current in a reversible manner (indicated by the horizontal bar). B. Representative current traces before and during application of 10 µM NNC 55-0396 dihydrochloride, as indicated.
      • Huang, L. et al. (2004) J. Pharmacol. Exp. Ther309, 193.
      • Chen, W.K. et al. (2010) J. Neurosci30, 10360.
      • NNC 55-0396 dihydrochloride, is a structural analog of mibefradil (Ro 40-5967) that inhibits both T-type and high-voltage-activated (HVA) Ca2+ channels with a higher selectivity for T-type Ca2+ channels. NNC 55-0396 exerts no effect against high voltage Ca2+ channels at 100 μM, but inhibits T-type channels in HEK293 cells with a potency comparable to that of mibefradil (IC50 values of 6.8 versus 10.1 μM)1. NNC 55- 0396 can be an essential tool in preventing human ovarian cancer cell proliferation as a result of its ability to inhibit the function of T-type Ca2+ channels. It is believed that NNC 55-0396 may function by dissolving in or passing through the plasma membrane of cells2.
    Target T-type CaV channels
    Last update: 24/06/2019

    NNC 55-0396 dihydrochloride (#N-205) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use