Overview
Type: Synthetic peptide
Form: Lyophilized powder
NrCAM (extracellular) Blocking Peptide (#BLP-NR045) is the original antigen used for immunization during Anti-NrCAM (extracellular) Antibody (#ANR-045) generation. The blocking peptide binds and ‘blocks’ Anti-NrCAM (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell (lanes 4 and 8) lysates:1-4. Anti-NrCAM (extracellular) Antibody (#ANR-045), (1:200).
5-8. Anti-NrCAM (extracellular) Antibody, preincubated with NrCAM (extracellular) Blocking Peptide (#BLP-NR045).
Expression of NrCAM in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NrCAM (extracellular) Antibody (#ANR-045), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus region, showed immunoreactivity (green) in neurons of the hilus (H, arrows). B. Pre-incubation of the antibody with NrCAM (extracellular) Blocking Peptide (BLP-NR045), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
Properties
Sequence
- (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM (Accession P97686).
Accession (Uniprot) Number P97686
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
