NrCAM (extracellular) Blocking Peptide (#BLP-NR045) is the original antigen used for immunization during Anti-NrCAM (extracellular) Antibody (#ANR-045) generation. The blocking peptide binds and ‘blocks’ Anti-NrCAM (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell (lanes 4 and 8) lysates:
- Expression of NrCAM in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NrCAM (extracellular) Antibody (#ANR-045), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus region, showed immunoreactivity (green) in neurons of the hilus (H, arrows). B. Pre-incubation of the antibody with NrCAM (extracellular) Blocking Peptide (BLP-NR045), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
- (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM (Accession P97686).