Overview
Type: Synthetic peptide
Form: Lyophilized powder
NRG1/Neuregulin-1 (extracellular) Blocking Peptide (#BLP-NR111) is the original antigen used for immunization during Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111) generation. The blocking peptide binds and ‘blocks’ Anti-NRG1/Neuregulin-1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart lysate (lanes 1 and 4), mouse heart membranes (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):1-3. Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111), (1:200).
4-6. Anti-NRG1 (Neuregulin-1) (extracellular) Antibody, preincubated with NRG1/Neuregulin-1 (extracellular) Blocking Peptide (#BLP-NR111).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 4), human U87-MG glioblastoma cell lysate (lanes 2 and 5) and human MDA-231 breast adenocarcinoma cell lysate (lanes 3 and 6):1-3. Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111), (1:200).
4-6. Anti-NRG1 (Neuregulin-1) (extracellular) Antibody, preincubated with NRG1/Neuregulin-1 (extracellular) Blocking Peptide (#BLP-NR111).
Expression of Neuregulin-1 in rat neocortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (#ANR-111), (1:1000), followed by anti-rabbit-biotinylated antibody and streptavidin-Cy3 (red). Neuregulin-1 staining is strong in processes of various cell types (horizontal arrows) and less intense in neuron-shaped soma (vertical arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KIQKKPGKSELRINK, corresponding to amino acid residues 87-101 of human Neuregulin-1 (Accession Q02297).
Accession (Uniprot) Number Q02297
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
