NRG2 (extracellular) Blocking Peptide (#BLP-NR114) is the original antigen used for immunization during Anti-NRG2 (extracellular) Antibody (#ANR-114) generation. The blocking peptide binds and ‘blocks’ Anti-NRG2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
- Expression of Neuregulin-2 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NRG2 staining in CA1 hippocampal region (green) is detected in the pyramidal layer (P, arrows). B. Staining in the cerebellum (green) is observed in Purkinje cells (arrows). Mol= Molecular layer; GL= Granular layer. Cell nuclei are stained with DAPI (blue).
- (C)RDIRIKYGNGRKNSR, corresponding to amino acid residues 297-311 of rat Neuregulin-2 (Accession O35569).