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Omega-conotoxin Bu8

Bu8, ω-Conotoxin Bu8

A Specific Blocker of CaV2.2 Channel

Cat #: STC-180
Alternative Name Bu8, ω-Conotoxin Bu8
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Conus bullatus (Bubble cone)
    Source Synthetic peptide
    MW: 2753 Da
    Purity: >98%
    Form Lyophilized Powder
    Effective concentration 10 – 100 nM
    Sequence CKRKGSSCRRTSYDCCTGSCRNGKC – NH2
    Modifications Disulfide bonds location- Cys1-Cys16, Cys8-Cys20, Cys15-Cys25
    Cys25 – C-terminal amidation
    Structure
    Molecular formula C103H174N42O35S6
    Activity Specific and potent N-type voltage-gated calcium (CaV2.2) channel blocker.
    References-Activity
    1. Chen, J. et al. (2021) Acta Pharm. Sin. B., 11, 2685.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Protect from light and moisture.
    Solubility Soluble in water. It is recommended to prepare fresh solutions in working buffers before use, or aliquot stock solutions reconstituted in distilled water and keep at -20°C. Upon use, dilute the stock solution in the desired working buffer. Prevent repeated thawing and freezing cycles. Centrifuge all product preparations before use (10,000 g for 1 min).
    Storage of solutions Store up to one week at 4°C or up to 6 months at -20°C
    Our bioassay
    • Alomone Labs ω-conotoxin Bu8 inhibits CaV2.2 channel currents heterologously expressed in Xenopus oocytes.
      Alomone Labs ω-conotoxin Bu8 inhibits CaV2.2 channel currents heterologously expressed in Xenopus oocytes.
      A. Representative time course of ω-conotoxin Bu8 (#STC-180) inhibition of CaV2.2 (α1B + α2δ1 + β1) channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage ramp to +60 mV every 10 sec, and inhibited by 10 nM ω-conotoxin Bu8 (green).
      B. Superimposed traces of CaV2.2 currents after application of control (black) and of 10 nM ω-conotoxin Bu8 (green), taken from the recording in A.
    References - Scientific background
    1. Chen, J. et al. (2021) Acta Pharm. Sin. B., 11, 2685.
    2. Adams, D.J. and Berecki, G. (2013) Biochim. Biophys. Acta. 1828, 1619.
    Scientific background

    ω-conotoxin Bu8 (Bu8) is a 25 amino acid peptidyl toxin that was synthesized from the venom of the cone snail, Conus bullatus1. This toxin potently and selectively blocks N-type voltage-gated calcium (Cav2.2) channels. Bu8 displays higher or similar analgesic activity in the pain models compared to MVIIA but has less adverse effects1.

    Bu8 adopts a canonical globular scaffold, demonstrated as an inhibitory cysteine knot motif, marked by 3 disulfide bonds that is consistent for all ω-conotoxins in the all-crossing pattern of 1-4, 2-5, and 3-61.

    Cav2.2 are predominantly expressed in nerve terminals and are involved in the regulation of neuronal excitability and nociceptive signaling. These channels are multifunctional and play important roles in the transduction of acute and chronic pain perception. They are able to transduce electrical activity into other cellular functions, regulate calcium homeostasis, and process pain information. Since ω-conotoxins selectively inhibit Cav2.2 in nociceptors, they are considered attractive molecules for drug design2.

    Target CaV2.2 Ca2+ channel
    Peptide Content: 100%
    Last update: 31/07/2022

    ω-Conotoxin Bu8 (#STC-180) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
    Shipping and Ordering information