Ononetin

A Potent TRPM3 Channel Blocker and a TRPA1 Channel Activator
Cat #: O-135
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 258.27
    Purity: >98%
    Effective concentration 10-100 µM.
    Chemical name 1-(2,4-dihydroxyphenyl)-2-(4-methoxyphenyl)ethanone.
    Molecular formula C15H14O4.
    CAS No.: 487-49-0.
    Activity Ononetin is a deoxybenzoin, a potent blocker of TRPM3 channels (IC50 = 0.3 µM), also shown to activate TRPA1 channels at 10-100 µM1.
      • Straub, I. et al. (2013) Br. J. Pharmacol. 168, 1835.

    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Protect from light.
    Solubility Soluble in DMSO and ethanol.
    Centrifuge all products before use (10000 x g 5 min).
    Storage of solutions Up to three months at -20°C.
      • Alomone Labs Ononetin activates TRPA1 channels expressed in HEK-293 cells.
        Dose response curve of the activation of human TRPA1 channels by increasing concentrations of Ononetin (#O-135). Cells were loaded with Calcium 5 dye. Changes in intracellular Ca2+ following Ononetin application were detected as changes in relative fluorescence (RFU). EC50 was determined at 19.7 µM.
      • Straub, I. et al. (2013) Br. J. Pharmacol. 168, 1835.
      • Held, K. et al. (2015) Temperature 2, 201.
      • Klionsky, L. et al. (2007) Mol. Pain 3, 39.
      • Ononetin is a deoxybenzoin that acts as a potent TRPM3 channel blocker with an IC50 of 0.3 µM and a TRPA1 channel activator between 10-100 µM. The compound binds at an extracellularly accessible binding site of the channel1.

        TRPM3, the third member of the melastatin-related TRPM channel subfamily, is a Ca2+-permeable non-selective cation channel expressed highly in the brain, spinal cord, sensory neurons, pituitary, kidney, eye, testis and adipose tissue. TRPM3 acts as a volume-regulated ion channel involved in the modulation of insulin release in pancreatic islets2.

        TRPA1, transient receptor potential ankyrin 1, is a non-selective cation channel expressed in the small neurons of the dorsal root and trigeminal ganglia. It plays an important role in models of inflammatory and neuropathic pain and have the ability to detect pungent or irritant compounds3.

    Target TRPM3, TRPA1 channels
    Last update: 17/01/2019

    Ononetin (#O-135) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use