P2X2 Receptor (extracellular) Blocking Peptide (#BLP-PR025) is the original antigen used for immunization during Anti-P2X2 Receptor (extracellular) Antibody (#APR-025) generation. The blocking peptide binds and ‘blocks’ Anti-P2X2 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat pheochromocytoma PC12 cells (lanes 3 and 6):
- (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653).