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Certificate of Analysis

P2X7 Receptor Deluxe Research Pack

All You Need for P2X7 Receptor Research

Cat #: ESD-710
Size: 12 Vials

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X7 Receptor Antibody Cat #: APR-004

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
Homology
Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.

Anti-P2X7 Receptor-ATTO Fluor-550 Antibody Cat #: APR-004-AO

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH Reactivity: H, M, R
Immunogen
Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
Homology
Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.
Label
ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Anti-P2X7 Receptor (extracellular) Antibody Cat #: APR-008

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Homology
Human, rat - identical; bovine - 14/17 amino acid residues identical.

Anti-P2X7 Receptor (extracellular)-FITC Antibody Cat #: APR-008-F

Size: 50 µl Host: Rabbit Type: Polyclonal Application: FC, IC, IF, LCI Reactivity: H, M, R
Immunogen
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Homology
Human, rat - identical; bovine - 14/17 amino acid residues identical.
Label
Fluorescein isothiocyanate (FITC).

Pharmacological Reagents

BzATP triethylammonium salt Cat #: A-385

Size: 10 mg Source: Synthetic MW: 1018.97 Purity: >95%
Effective concentration
0.5-300 µM.
Activity
BzATP triethylammonium salt is an agonist of the purinergic P2X7 receptor, more potent than ATP, with EC50 varying from 0.7 to 285 µM, depending on receptor species, buffer composition, serum constituents and repeated exposure to ATP. Shows partial agonist activity on other P2X receptors. Can be used as a photoaffinity label for ATPase1-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble up to 100 mM in water. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

A-740003 Cat #: A-420

Size: 50 mg Source: Synthetic MW: 474.55 Purity: >96% (HPLC)
Effective concentration
10 nM – 1 µM.
Activity
A-740003 is a potent and selective competitive antagonist of P2X7 receptors with IC50 values of 18 nM, 44 nM and 0.68 µM for rat, human and mouse receptors, respectively. A-740003 reduces neuropathic pain and tumor growth in vivo1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in DMSO. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

A-804598 Cat #: A-415

Size: 25 mg Source: Synthetic MW: 315.37 Purity: >99%
Effective concentration
1-100 nM.
Activity
A-804598 is a potent and selective, competitive and high-affinity antagonist of the purinergic P2X7 receptor, with IC50 values of around 10 nM for mouse, rat, and human receptors1-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in DMSO. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
Store at -20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

JNJ-47965567 Cat #: J-115

Size: 25 mg Source: Synthetic MW: 488.65 Purity: >99% (HPLC)
Effective concentration
20 nM – 1 µM.
Activity
JNJ-47965567 is a potent and highly selective antagonist of purinergic P2X7 receptors, shown to inhibit BzATP-induced Ca2+ responses at human, rat and mouse P2X7 receptors with pIC50 values of 8.3, 7.2 and 7.5, respectively. JNJ-47965567 is centrally permeable and active in vivo1.
Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at +4°C.

Reconstitution
Soluble in DMSO. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

KN-62 Cat #: K-120

Size: 10 mg Source: Synthetic MW: 721.85 Purity: >98%
Effective concentration
20 nM – 20 µM.
Activity
KN-62 is a potent non-competitive antagonist of the purinergic P2X7 receptors, shown to inhibit Bz-ATP-induced currents in HEK–hP2X7 cells with IC50 of 25 nM. KN-62 is also a selective, cell permeable inhibitor of Ca2+/calmodulin-dependent kinase type II, with IC50 of approximately 1 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in DMSO. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

ZINC9315614 Cat #: Z-145

Size: 25 mg Source: Synthetic MW: 328.74 Purity: >98%
Effective concentration
0.1-10 µM.
Activity
ZINC9315614 is a novel antagonist of the purinergic P2X7 receptors, shown to inhibit YoPro1 uptake and BzATP-induced Ca2+ responses in HEK–hP2X7 cells with IC50 of 0.8 µM and 3.2 µM, respectively1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble up to 100 mM in DMSO. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

Garcinolic acid Cat #: G-155

Size: 1 mg Source: Natural MW: 646.78 Purity: >95%
Origin
Garcinia spp.
Effective concentration
10-100 µM.
Activity
Garcinolic acid is a natural compound isolated from Garcinia resin, shown to facilitate and potentiate cellular responses mediated by the purinergic P2X7 receptors at over 10 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble in DMSO, DMF and other organic solvents. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

GW 791343 hydrochloride Cat #: G-115

Size: 25 mg Source: Synthetic MW: 483.81 Purity: >99%
Effective concentration
0.1-10 µM.
Activity
GW 791343 hydrochloride is a species-specific allosteric modulator of the purinergic P2X7 receptors, acting as a negative modulator of human P2X7 (pIC50 = 6.9 - 7.2), and as a positive modulator of rat P2X71,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Soluble up to 100 mM in DMSO and in water. Centrifuge all products before handling (10000 x g 5 min).
Storage after reconstitution
-20°C. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use
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