Overview
Type: Synthetic peptide
Form: Lyophilized powder
P2Y4 Receptor Blocking Peptide (#BLP-PR006) is the original antigen used for immunization during Anti-P2Y4 Receptor Antibody (#APR-006) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y4 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-P2Y4 Receptor Antibody (#APR-006), (1:300).
2. Anti-P2Y4 Receptor Antibody, preincubated with P2Y4 Receptor Blocking Peptide (#BLP-PR006).
Expression of P2RY4 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-P2Y4 Receptor Antibody (#APR-006). A. P2RY4 (red) appears in astrocytic processes (arrows). B. In contrast, P2RY4 does not appear in Purkinje neuronal dendrites stained for calbindin D28k (green) in the same brain section. This demonstrates the restriction of P2RY4 to astrocytic fibers.- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
Properties
Sequence
- (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811).
Accession (Uniprot) Number O35811
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
