Overview
Type: Synthetic peptide
Form: Lyophilized powder
Pan ASIC (extracellular) Blocking Peptide (#BLP-SC031) is the original antigen used for immunization during Anti-pan ASIC (extracellular) Antibody (#ASC-031) generation. The blocking peptide binds and ‘blocks’ Anti-Pan ASIC (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:500).
3-4. Anti-pan ASIC (extracellular) Antibody, preincubated with Pan ASIC (extracellular) Blocking Peptide (#BLP-SC031).
Western blot analysis of mouse brain membranes (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysate (lanes 2 and 4):1-2. Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:500).
3-4. Anti-pan ASIC (extracellular) Antibody, preincubated with Pan ASIC (extracellular) Blocking Peptide (#BLP-SC031).
Expression of ASIC channels in rat locus coeruleusImmunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. ASIC channel staining (green) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1 (Accession P55926).
Accession (Uniprot) Number P55926
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
