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Certificate of Analysis

Parkinson’s Disease Marker Antibody Kit

A Screening Package of Parkinson’s Disease Marker Antibodies Economically Priced

Cat #: AK-580
Size: 30 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-CDNF Antibody Cat #: ANT-027

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M
Immunogen
Peptide (C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF (Accession Q49AH0). Mature CDNF protein.
Homology
Mouse - 10/13 amino acid residues identical.

CDNF Blocking Peptide Cat #: BLP-NT027

Size: 40 µg

Anti-Connexin-43 Antibody Cat #: ACC-201

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.
Homology
Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.

Connexin-43 Blocking Peptide Cat #: BLP-CC201

Size: 40 µg

Anti-D1 Dopamine Receptor Antibody Cat #: ADR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.
Homology
Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.

D1 Dopamine Receptor Blocking Peptide Cat #: BLP-DR001

Size: 40 µg

Guinea pig Anti-D1 Dopamine Receptor Antibody Cat #: ADR-001-GP (formerly AGP-100)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 (Accession P18901). Intracellular, C-terminus.
Homology
Mouse, dog, pig - identical; human - 13/14 amino acid residues identical.

D1 Dopamine Receptor Blocking Peptide Cat #: BLP-DR001

Size: 40 µg

Anti-D2 Dopamine Receptor (extracellular) Antibody Cat #: ADR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DDLERQNWSRPFNGSE, corresponding to amino acid residues 11-26 of rat DRD2 (Accession P61169). Extracellular, N-terminus.
Homology
Mouse - identical; human - 15/16 amino acid residues identical.

D2 Dopamine Receptor (extracellular) Blocking Peptide Cat #: BLP-DR002

Size: 40 µg

Anti-Dopamine Transporter (DAT) (extracellular) Antibody Cat #: AMT-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)DAHASNSSDGLGLND, corresponding to amino acids residues 191-205 of rat DAT (Accession P23977). 2nd extracellular loop.
Homology
Mouse - 14/15 amino acid residues identical.

Dopamine Transporter/DAT (extracellular) Blocking Peptide Cat #: BLP-MT003

Size: 40 µg

Anti-GDNF Antibody Cat #: ANT-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905). C-terminus of the mature GDNF.
Homology
Mouse, dog, monkey, bovine - identical; rat 15/16 amino acid residues identical.

GDNF Blocking Peptide Cat #: BLP-NT014

Size: 40 µg

Anti-GIRK2 (Kir3.2) Antibody Cat #: APC-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2 (Accession P48542). Intracellular, C-terminal part.
Homology
Rat - 40/41 amino acid residues identical; golden hamster - 39/41 amino acid residues identical; human - 37/41 amino acid residues identical.

GIRK2/Kir3.2 Blocking Peptide Cat #: BLP-PC006

Size: 0.12 mg

Guinea pig Anti-GIRK2 (Kir3.2) Antibody Cat #: APC-006-GP (formerly AGP-013)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2 (GIRK2) (Accession P48542), Intracellular, C-terminal part. (MW: 31 kDa. is the size of the fusion protein).
Homology
Rat - 40/41, human – 37/41  amino acid residues identical.

GIRK2/Kir3.2 Blocking Peptide Cat #: BLP-PC006

Size: 0.12 mg

Anti-GPR37 (extracellular) Antibody Cat #: AGR-049

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, WB Reactivity: H, M, R
Immunogen
Peptide (C)RVRLKNPFYPLTQE, corresponding to amino acid residues 234-247 of rat GPR37 (Accession Q9QYC6). Extracellular, N-terminus.
Homology
Mouse, human – identical.

GPR37 (extracellular) Blocking Peptide Cat #: BLP-GR049

Size: 40 µg

Anti-Homer1 Antibody Cat #: APZ-026

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1 (Accession Q9Z214). Intracellular.
Homology
Mouse, human – identical.

Homer1 Blocking Peptide Cat #: BLP-PZ026

Size: 40 µg

Anti-LRRK2 Antibody Cat #: ANR-102

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CKALHVLFERVSEE, corresponding to amino acid residues 183-196 of mouse LRRK2 (Accession Q5S006). N-terminus.
Homology
Rat, human  – identical.

LRRK2 Blocking Peptide Cat #: BLP-NR102

Size: 40 µg

Anti-MANF/ARMET Antibody Cat #: ANT-028

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF (Accession P55145). Mature MANF protein.
Homology
Rat, mouse - identical.

MANF/ARMET Blocking Peptide Cat #: BLP-NT028

Size: 40 µg

Anti-Alpha-Synuclein Antibody Cat #: APZ-035

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)KDQMGKGEEGYPQE, corresponding to amino acid residues 97-110 of rat α-synuclein (Accession P37377). Intracellular, C-terminus.
Homology
Mouse – identical; human – 11/14 amino acid residues identical.

Alpha-Synuclein Blocking Peptide Cat #: BLP-PZ035

Size: 40 µg

Anti-VMAT2 Antibody Cat #: AMT-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2 (Accession Q01827). 1st luminal loop.
Homology
Rat, mouse, human – 12/13 amino acid residues identical.

VMAT2 Blocking Peptide Cat #: BLP-MT006

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#ADR-001-GP & #APC-006-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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