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Parthenolide

A Nuclear Factor-κB (NF-κB) Pathway Inhibitor

Cat #: P-250
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Chrysanthemum parthenium.
    Source Natural
    MW: 248.3
    Purity: >99%
    Effective concentration 10-50 µM.
    Structure
    Chemical name (1aR,7aS,10aS)- 1a,5-dimethyl-8-methylene- 2,3,6,7,7a,8,10a,10b- octahydrooxireno[9,10]cyclodeca [1,2-b]furan-9(1aH)-one.
    Molecular formula C15H20O3.
    CAS No.: 20554-84-1
    Activity Parthenolide is a sesquiterpene lactone and inhibits IκB kinase (IKK) and IKKβ (the upstream activators of the Nuclear Factor-κB (NF-κB) pathway)1,2.
    References-Activity
    1. Lee, K.H. et al. (1977) Science 196, 533.
    2. Bork, P.M. et al. (1997) FEBS Lett402, 85.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO, ethanol, methanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or six months at -20°C.
    Our bioassay
    • Alomone Labs Parthenolide inhibits the NF-κB pathway in Jurkat and HeLa cells.
      Alomone Labs Parthenolide inhibits the NF-κB pathway in Jurkat and HeLa cells.
      Jurkat (A) and HeLa (B) cells were treated with different concentrations of Parthenolide (#P-250) for 1 h. Jurkat cells were then stimulated with 100 nM PMA (#P-800) and 1 µM Ionomycin (#I-700) for 15 min. Hela cells were stimulated with 4000 U/ml TNFα for 10 min. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-p65 NF-κB.
    References - Scientific background
    1. Johnson, E.S. et al. (1985) Br. Med. J. 291, 569.
    2. Biggs, M.J. et al. (1982) Lancet 2, 776.
    3. Hall, I.H. et al. (1979) J. Pharmacol. Sci. 68, 537.
    4. Douros, J. and Suffness, M. (1978) Cancer Chemother. Pharmacol. 1, 91.
    5. Lee, K.H. et al. (1977) Science 196, 533.
    6. Bork, P.M. et al. (1997) FEBS Lett. 402, 85.
    7. Hehner, S.P. et al. (1999) J. Immunol. 163, 5617.
    8. Patel, N.M. et al. (2000) Oncogene 19, 4159.
    9. Wang, W. et al. (1999) Clin. Cancer Res. 5, 119.
    10. Wen, J. et al. (2002) J. Biol. Chem277, 38954.
    Scientific background

    Parthenolide is a sesquiterpene lactone found in Mexican-Indian medicinal plants such as Tanacetum parthenium. It has been widely used in indigenous medical practices for the treatment of migraine, inflammation and tumors.1-4 Parthenolide inhibits IkB kinase (IKK) and IKKβ (the upstream activators of the Nuclear Factor-kB (NF-kB) pathway), thus inhibiting Interleukin 1- and tumor necrosis factor a (TNFα) without affecting the activation of p38 and c-Jun N-terminal kinase.5,6

    In breast adenocarcinoma and pancreatic tumor cell lines which constitutively express active NF-κB, parthenolide inhibits NF-κB DNA binding and increases sensitivity to chemotherapeutic agents. The sensitivity of these tumor cells to parthenolide is due to the low expression level of the multifunctional detoxification enzyme glutathione S-transferase p caused by inhibition of NF-κB activity.7,10

    Last update: 11/04/2021

    Parthenolide (#P-250) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use

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