Every lot is tried & tested in a relevant biological assay.
- Huang, Q. et al. (2014) J. Med. Chem. 57, 1170.
- Alomone Labs PF-06439015 inhibits BDNF-induced ERK1/2 (p42/44 MAPK) phosphorylation in TrkB-expressing HEK 293 cells.Serum-deprived cells were stimulated with 10 ng/ml Recombinant human BDNF protein (#B-250) in the presence of 0 nM, 8 nM, 80 nM and 800 nM PF-06439015 (#P-355). Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-ERK1/2.
PF-06439015 is a synthetic compound that acts as a potent inhibitor of receptor tyrosine kinases. It inhibits various tyrosine kinases including ALK, ROS1, FER, LTK and TrkB with IC50 values of less than 10 nM1.
Tyrosine kinases are a family of enzymes that acts as important mediators of the signaling cascade leading to cell proliferation, differentiation, migration, metabolism, and programmed cell death. Tyrosine kinases catalyze phosphorylation of select tyrosine residues in target proteins2.