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Certificate of Analysis

pH-Sensitive TRP Channel Antibody Explorer Kit

A Screening Package of pH-Sensitive TRP Channel Antibodies Economically Priced

Cat #: AK-475
Size: 7 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPV1 (VR1) Antibody Cat #: ACC-030

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Homology
Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.

Anti-Rat TRPV1 (VR1) (extracellular) Antibody Cat #: ACC-029

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: R
Immunogen
Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
Homology
Specific to rat TRPV1.

Anti-TRPV4 Antibody Cat #: ACC-034

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.
Homology
Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

Anti-TRPV4 (extracellular) Antibody Cat #: ACC-124

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, R
Immunogen
Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Homology
Rat - 14/16 amino acid residues identical; mouse - 13/16 amino acid residues identical; human - 12/16 amino acid residues identical.

Anti-TRPC4 Antibody Cat #: ACC-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5). Intracellular, C-terminus.
Homology
Rat, human - 15/16 amino acid residues identical; bovine -  11/15 amino acid residues identical.

Anti-TRPC4 (extracellular) Antibody Cat #: ACC-119

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4 (Accession O35119). 2nd extracellular loop.
Homology
Mouse, human - identical.

Anti-TRPC5 Antibody Cat #: ACC-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5 (Accession Q9UL62). Intracellular, C-terminus.
Homology
Rat, mouse, rabbit - identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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