Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Platelet-activating factor receptor (extracellular) Blocking Peptide (#BLP-PR055) is the original antigen used for immunization during Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055) generation. The blocking peptide binds and ‘blocks’ Anti-Platelet-activating factor receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500).
3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody, preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500).
3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody, preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).
Expression of Platelet-activating factor receptor in rat substantia nigra pars compacta (SNC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PAFR immunoreactivity (green) appears in SNC neurons (arrows). B. Pre-incubation of the antibody with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR (Accession Q62035).
Accession (Uniprot) Number Q62035
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
