PNU-120596

A Potent and Selective Positive Allosteric Modulator of α7 nAChR
Cat #: P-350
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 311.72
    Purity: >98% (HPLC)
    Effective concentration 0.3-10 µM.
    Chemical name 1-(5-chloro-2,4-dimethoxyphenyl)-3-(5-methyl-1,2-oxazol-3-yl)urea.
    Molecular formula C13H14ClN3O4.
    CAS No.: 501925-31-1.
    PubChem CID 311434
    Activity PNU-120596 is a potent and selective type-II positive allosteric modulator of α7 nicotinic acetylcholine receptors, shown to potentiate α7 currents in X. Laevis oocytes and in SH-EP1 cells with EC50 values of 1.6 µM and 216 nM, respectively1-2. Neuroprotective in vivo3.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at +4°C.
    Solubility Soluble to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Store at -20°C. It is recommended to prepare solutions afresh on the day of use, or aliquot stock solutions stored at -20°C to prevent repeated thawing and freezing.
      • Alomone Labs PNU-120596 positively modulates α7 nAChR expressed in Xenopus oocytes.
        A. Representative time course of PNU-120596 (#P-350) effect on α7 current, elicited every 100 sec by transient applications of 50 µM acetylcholine, while membrane potential was held at -80 mV. Current significantly enhanced after a 60 sec pre-application of 3 µM PNU-120596 (green bar). B. Superimposed traces of α7 currents before (black) and after (green) application of 3 µM PNU-120596, which enhanced the current evoked by acetylcholine stimulation (arrow). Taken from the experiment in A.
      • PNU-120596 is as a potent and selective positive allosteric modulator of α7 nicotinic acetylcholine receptors (nAChRs). It potentiates nAChR α7 currents in Xenopus oocytes and in SH-EP1 cells with EC50 values of 1.6 µM and 216 nM, respectively1-2. The compound displays neuroprotective characteristics in vivo and has the ability to increase the potency and maximal efficacy of agonists and to prolong the duration of the agonist-evoked macroscopic currents1,2.

        nAChRs receptors are responsible for mediating the effects of the neurotransmitter acetylcholine (ACh). They play critical roles in the central and peripheral nervous system where they regulate neurotransmitter release, cell excitability, neuronal integration. They are involved in functions such as sleep and arousal patterns, fatigue, hunger, anxiety, and pain processing2,3,4.

    Target α7 nAChR
    Last update: 18/07/2019

    PNU-120596 (#P-350) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use