Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prostaglandin E Receptor EP1/PTGER1 (extracellular) Blocking Peptide (#BLP-PR063) is the original antigen used for immunization during Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody (#APR-063) generation. The blocking peptide binds and ‘blocks’ Anti-Prostaglandin E Receptor EP1/PTGER1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat colon lysate (lanes 1 and 4), mouse kidney lysate (lanes 2 and 5) and human HT-29 colon adenocarcinoma cell lysate (lanes 3 and 6):1-3. Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody (#APR-063), (1:200).
4-6. Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP1/PTGER1 (extracellular) Blocking Peptide (#BLP-PR063).
Properties
Sequence
- (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375).
Accession (Uniprot) Number P35375
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
