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Certificate of Analysis

Prostanoid Receptor Antibody Explorer Kit

A Screening Package of Prostanoid Receptor Antibodies Economically Priced

Cat #: AK-655
Size: 8 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-CRTH2/GPR44 (extracellular) Antibody Cat #: APR-062

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2 (Accession Q6XKD3). 3rd extracellular loop.
Homology
Mouse – identical. Will not recognize DP2 receptor from human samples.

Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody Cat #: APR-068

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, WB Reactivity: H, M, R
Immunogen
Peptide (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR (Accession P43253). Extracellular, 3rd loop.
Homology
Mouse – identical; human – 11/13 amino acid residues identical.

Anti-Prostaglandin E Receptor EP1 (PTGER1) (extracellular) Antibody Cat #: APR-063

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)GPDDRRRWGSRGPR, corresponding to amino acid residues 249-262 of mouse prostanoid EP1 receptor (Accession P35375). 3rd extracellular loop.
Homology
Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody Cat #: APR-064

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928). 3rd extracellular loop.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody Cat #: APR-065

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, WB Reactivity: H, M, R
Immunogen
Peptide (C)RAPHWYASHMKTR, corresponding to amino acid residues 137-149 of mouse prostanoid EP3 receptor (Accession P34980). 2nd intracellular loop.
Homology
Mouse, human – identical.

Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody Cat #: APR-066

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, WB Reactivity: H, M, R
Immunogen
Peptide (C)RKTVLSKAIEKIK, corresponding to amino acid residues 336-348 of rat prostanoid EP4 receptor (Accession P43114). Intracellular, C-terminus.
Homology
Mouse, human – identical.

Anti-Prostaglandin F2-α Receptor/PTGFR Antibody Cat #: APR-067

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RSQQHRQGRSHHLE, corresponding to amino acid residues 233-246 of rat Prostaglandin F2-α receptor (Accession P43118). Intracellular, 3rd loop.
Homology
Mouse – identical; human – 13/14 amino acid residues identical.

Anti-Thromboxane A2 Receptor Antibody Cat #: APR-069

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225-239 of rat TBXA2R (Accession P34978). 3rd intracellular loop.
Homology
Rat – 14/15 amino acid residues identical; mouse – 13/15 amino acid residues identical; human – 12/15 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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