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Certificate of Analysis

Membrane Proteins in Inflammation Antibody Explorer Kit

A Screening Package of Inflammatory Membrane Protein Antibodies Economically Priced

Cat #: AK-605
Size: 17 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X1 Receptor (extracellular) Antibody Cat #: APR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.
Homology
Rat, mouse - 11/14 amino acid residues identical.

Anti-P2X7 Receptor (extracellular) Antibody Cat #: APR-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Homology
Human, rat - identical; bovine - 14/17 amino acid residues identical.

Anti-GPR65 (TDAG8) (extracellular) Antibody Cat #: AGR-043

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65 (Accession Q8IYL9). 1st extracellular loop.
Homology
Mouse – 12/13 amino acid residues identical; rat - 11/13 amino acid residues identical.

Anti-BAI1 (extracellular) Antibody Cat #: ABR-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)GRVRTYQFDSFLESTR, corresponding to amino acid residues 97-112 of mouse BAI1 (Accession Q3UHD1). Extracellular, N-terminus.
Homology
Rat, human - identical

Anti-β2-Adrenergic Receptor (extracellular) Antibody Cat #: AAR-016

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ADRB2 (Accession P18762). Extracellular, N-terminus.
Homology
Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical.

Anti-Calcium Sensing Receptor (extracellular) Antibody Cat #: ACR-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR (Accession P41180). N-terminus, extracellular.
Homology
Rat, mouse, bovine, canis - identical.

Anti-Human BLT1 (extracellular) Antibody Cat #: ALR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, WB Reactivity: H
Immunogen
Peptide CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R (Accession Q15722). 2nd extracellular loop.
Homology
Monkey - identical.

Anti-Human C5aR1 (extracellular) Antibody Cat #: AAR-032

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H
Immunogen
Peptide (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor (Accession P21730). Extracellular, N-terminus.
Homology
Human only. Not recommended for use with rat and mouse samples.

Anti-CysLTR1 (extracellular) Antibody Cat #: ALR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, WB Reactivity: H
Immunogen
Peptide (C)HNETKP(S)DSVLRMQK, corresponding to amino acid residues 261-275 of human CysLTR1 (Accession Q9Y271). 3rd extracellular loop.
Homology
Human - 14/15; mouse - 12/15 amino acid residues identical; rat - 11/15 amino acid residues identical.

Anti-Human FPR2/ALX (extracellular) Antibody Cat #: AFR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H
Immunogen
Peptide (C)GGTPEERLKVAIT, corresponding to amino acid residues 184-196 of human FPR2/ALX (Accession P25090). 2nd extracellular loop.
Homology
Human only.

Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody Cat #: AHR-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H
Immunogen
Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4 (Accession Q9H3N8). 1st extracellular loop.
Homology
Won't recognize rat or mouse H4 Histamine Receptor.

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody Cat #: APR-064

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928). 3rd extracellular loop.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Anti-S1PR1 (EDG1) (extracellular) Antibody Cat #: ASR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1 (Accession P21453). Extracellular, N-terminus.
Homology
Mouse, rat - 13/14 amino acid residues identical.

Anti-S1PR3 (EDG3) (extracellular) Antibody Cat #: ASR-013

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3 (Accession Q9Z0U9). Extracellular, N-terminus.
Homology
Rat - identical; human - 9/12 amino acid residues identical.

Anti-EMR1 (ADGRE1) (extracellular) Antibody Cat #: AER-051

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.
Homology
Mouse, Rat –11/14 amino acid residues identical.

Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody Cat #: ASR-033

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A (Accession P14842). Extracellular, N-terminus.
Homology
Mouse - identical; human - 9/13 amino acid residues identical.

Anti-xCT/SLC7A11 (extracellular) Antibody Cat #: ANT-111

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter (Accession Q9WTR6). 3rd extracellular loop.
Homology
Rat – identical; human – 11/14 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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