Every lot is tried & tested in a relevant biological assay.
- Alomone Labs ProTx-I inhibits NaV1.5 currents in stably transfected HEK293 cells.A. Time course of ProTx-I (#STP-400) action. Current amplitudes were plotted as a function of time. Holding potential was -100 mV and currents were stimulated every 10 seconds by a voltage ramp of 50 msec from holding potential to +60 mV. 50 nM ProTx-I was perfused in the period marked by the horizontal bar (green). B. Superimposed traces of NaV1.5 currents before and during 3 min application of 50 nM ProTx-I.
- Alomone Labs ProTx-I inhibits CaV3.1 channels expressed in Xenopus oocytes.A. Representative time course of ProTx-I (#STP-400) inhibition of CaV3.1 maximum current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage step to -30 mV every 10 sec, and was significantly inhibited by application of 1 µM ProTx-I (top horizontal bar). B. Superimposed traces of CaV3.1 current following the application of control and of 1 µM ProTx-I (as indicated). Taken from experiment in A.
Native ProTx-I has been isolated from the venom of the tarantula Thrixopelma pruriens. It was purified on the basis of its ability to reversibly inhibit the tetrodotoxin (TTX)-resistant Na channel NaV1.8. ProTx-I was also found to be an inhibitor of NaV1.5, NaV1.7 and NaV1.3 with IC50 values below 100 nM. Furthermore, ProTx-I shifts the voltage dependence activity of CaV3.1 (α1G) (IC50 ~50 nM) without affecting the voltage dependence of inactivation1,2. ProTx-I also blocks TRPA1 channel with an IC50 of 0.39 µM3.
Structurally, ProTx-I is shown to belong to the inhibitory cystine knot (ICK) family of peptidyl toxins interacting with voltage-gated ion channels.
Alomone Labs ProTx-I and ProTx-II inhibit T-type CaV channels.ProTx-I (#STP-400) was applied to mouse thalamic neurons and ProTx-II (#STP-100) was tested on mouse DRGs. ProTx-I blocks native mouse CaV3.1 channel and recombinant human CaV3.1 channel currents similarly, and blocks to a lesser extent CaV3.2 and CaV3.3 channel currents. ProTx-II blocks CaV3.2 channels more potently than the other T-type currents.Adapted from Bladen, C. et al. (2014) Mol. Brain 7, 36. with permission of BioMed Central.
ProTx-I (#STP-400) is a highly pure, synthetic, and biologically active peptide toxin.
- Bladen, C. et al. (2014) Mol. Brain 7, 36.
- Anti-NaV1.7 (SCN9A) Antibody (#ASC-008)
- Anti-NaV1.8 (SCN10A) Antibody (#ASC-016)
- Anti-CACNA1G (CaV3.1) Antibody (#ACC-021)
- Anti-KV2.1 Antibody (#APC-012)
- Anti-TRPA1 (extracellular) Antibody (#ACC-037)
- ProTx-II (#STP-100)
- ProTx-II-Biotin (#STP-100-B)
- ProTx-III (#STP-150)
- Huwentoxin-IV (#STH-100)
- Kurtoxin (#STK-800)
- NaV Channel Blockers for Pain Research Explorer Kit (#EK-375)
- TTX-Sensitive NaV Channel Blocker Explorer Kit (#EK-105)
- TTX-Resistant NaV Channel Blocker Explorer Kit (#EK-106)
- T-Type CaV Channel Blocker Explorer Kit (#EK-111)
- KV2 Channel Blocker Explorer Kit (#EK-220)
- TRPA1 Channel Blocker Explorer Kit (#EK-121)
- TRPA1 Channel Modulator Explorer Kit (#EK-120)
- Shaker (KV)-Related Channel Blocker Explorer Kit (#EK-108)