Cat #: BLP-PR051
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-PTH1R (extracellular) Antibody (#APR-051). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates:1-3. Anti-PTH1R (extracellular) Antibody (#APR-051), (1:200).
4-6. Anti-PTH1R (extracellular) Antibody, preincubated with PTH1R (extracellular) Blocking Peptide (#BLP-PR051).
- Expression of Parathyroid hormone receptor 1 in rat brainImmunohistochemical staining of rat dorsal Raphe nucleus using Anti-PTH1R (extracellular) Antibody (#APR-051). A. PTH1R (red) staining is detected in Raphe neurons (arrows). B. Nucleus staining using DAPI as the counterstain (blue). C. Merged images of A and B.
- Multiplex staining of Parathyroid hormone receptor 1 and Orexin receptor 1 in rat ventromedial hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG), (1:60) and Anti-PTH1R (extracellular) Antibody (#APR-051), (1:60). A. Sections were incubated with Anti-PTH1R (extracellular) Antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody, (green). C. Merge of A and B shows staining of Orexin receptor 1 in nerve fibers (vertical arrows) while Parathyroid hormone receptor 1 is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat PTH1R (Accession P25961).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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