Overview
Type: Synthetic peptide
Form: Lyophilized powder
PTH1R (extracellular) Blocking Peptide (#BLP-PR051) is the original antigen used for immunization during Anti-PTH1R (extracellular) Antibody (#APR-051) generation. The blocking peptide binds and ‘blocks’ Anti-PTH1R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates:1-3. Anti-PTH1R (extracellular) Antibody (#APR-051), (1:200).
4-6. Anti-PTH1R (extracellular) Antibody, preincubated with PTH1R (extracellular) Blocking Peptide (#BLP-PR051).
Expression of Parathyroid hormone receptor 1 in rat brainImmunohistochemical staining of rat dorsal Raphe nucleus using Anti-PTH1R (extracellular) Antibody (#APR-051). A. PTH1R (red) staining is detected in Raphe neurons (arrows). B. Nucleus staining using DAPI as the counterstain (blue). C. Merged images of A and B.
Multiplex staining of Parathyroid hormone receptor 1 and Orexin receptor 1 in rat ventromedial hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG), (1:60) and Anti-PTH1R (extracellular) Antibody (#APR-051), (1:60). A. Sections were incubated with Anti-PTH1R (extracellular) Antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody, (green). C. Merge of A and B shows staining of Orexin receptor 1 in nerve fibers (vertical arrows) while Parathyroid hormone receptor 1 is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat PTH1R (Accession P25961).
Accession (Uniprot) Number P25961
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
