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Rabbit IgG Isotype Control-APC

Cat #: RIC-001-APC
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified form non-immunized rabbits.
Label Allophycocyanin (APC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Flow cytometry
Applications: fc
Negative control in surface staining flow cytometry
Direct flow cytometry
  • Establishing the effectiveness of the Rabbit IgG Isotype Control-APC (#RIC-001-APC) control alongside cell surface detection of monocarboxylate transporter 1 by direct flow cytometry in a live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:
    Establishing the effectiveness of the Rabbit IgG Isotype Control-APC (#RIC-001-APC) control alongside cell surface detection of monocarboxylate transporter 1 by direct flow cytometry in a live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC), (1µg).
    ___ Cells + Anti-MCT1 (SLC16A1) (extracellular)-APC Antibody (#AMT-011-APC), (1µg).
  • Establishing the effectiveness of the Rabbit IgG Isotype Control-APC (#RIC-001-APC) control alongside cell surface detection of PAR4 by direct flow cytometry in live intact human MEG-01 megakaryocytic cell line:
    Establishing the effectiveness of the Rabbit IgG Isotype Control-APC (#RIC-001-APC) control alongside cell surface detection of PAR4 by direct flow cytometry in live intact human MEG-01 megakaryocytic cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC), (1µg).
    ___ Cells + Anti-PAR4 (F2RL3) (extracellular)-APC Antibody (#APR-034-APC), (1µg).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Last update: 03/07/2022

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-APC (#RIC-001-APC), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Allophycocyanin (APC).

For research purposes only, not for human use

Applications

Specifications

Scientific Background

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