RAGE (extracellular) Blocking Peptide (#BLP-NR176) is the original antigen used for immunization during Anti-RAGE (extracellular) Antibody (#ANR-176) generation. The blocking peptide binds and ‘blocks’ Anti-RAGE (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
- Western blot analysis of human PANC-1 pancreatic carcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):
- Peptide (C)GKETKSNYRVRVYQIPGK, corresponding to amino acid residues 106 - 123 of human RAGE (Accession Q15109).