RAMP1 (extracellular) Blocking Peptide (#BLP-RR021) is the original antigen used for immunization during Anti-RAMP1 (extracellular) Antibody (#ARR-021) generation. The blocking peptide binds and ‘blocks’ Anti-RAMP1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat pancreas (lanes 3 and 6):
- Expression of RAMP1 in rat brainImmunohistochemical staining of rat free-floating frozen section using Anti-RAMP1 (extracellular) Antibody (#ARR-021). A. In the parietal cortex, RAMP1 immunoreactivity (green) appears in pyramidal neurons (arrows). B. In the hippocampus, RAMP1 staining (green) is detected in the CA1 region (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
- CRDPDYGTLIQE, corresponding to amino acid residues 27-38 of rat RAMP1 (Accession Q9JJ74).