RAMP2 (extracellular) Blocking Peptide (#BLP-RR022) is the original antigen used for immunization during Anti-RAMP2 (extracellular) Antibody (#ARR-022) generation. The blocking peptide binds and ‘blocks’ Anti-RAMP2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 4, 1:200), mouse heart (lanes 2 and 5, 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6, 1:400) lysates:
- Western blot analysis of rat spleen (lanes 1 and 3) and cortex (lanes 2 and 4):
- (C)HPTEDSLLSKGKMED, corresponding to amino acid residues 54-68 of rat RAMP2 (Accession Q9JHJ1).