Ret (extracellular) Blocking Peptide (#BLP-NT025) is the original antigen used for immunization during Anti-Ret (extracellular) Antibody (#ANT-025) generation. The blocking peptide binds and ‘blocks’ Anti-Ret (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
- Expression of Ret in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Ret (extracellular) Antibody (#ANT-025), (1:50). Ret staining (red) is detected in DRG neurons. Hoechst 33342 (blue) is used to stain cell nuclei.
- CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret (Accession P07949).