Ryanodine Receptor 3 Blocking Peptide (#BLP-RR003) is the original antigen used for immunization during Anti-Ryanodine Receptor 3 Antibody (#ARR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Ryanodine Receptor 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of RyR3 in rat cortexImmunohistochemical staining of rat brain frozen sections with Anti-Ryanodine Receptor 3 Antibody (#ARR-003), (red), (1:100). RyR3 is widely expressed in neurons of the cortex. Hoechst 33342 is used as the counterstain.
- CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3 (Accession Q15413).