Overview
Type: Synthetic peptide
Form: Lyophilized powder
SCN3A/Nav1.3 (extracellular) Blocking Peptide (#BLP-SC023) is the original antigen used for immunization during Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023) generation. The blocking peptide binds and ‘blocks’ Anti-SCN3A/Nav1.3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat newborn brain lysates (lanes 1 and 3), rat adult brain membranes (lane 2):1,2. Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023), (1:200).
3. Anti-SCN3A (NaV1.3) (extracellular) Antibody, preincubated with SCN3A/Nav1.3 (extracellular) Blocking Peptide (#BLP-SC023).
Properties
Sequence
- CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3 (Accession P08104).
Accession (Uniprot) Number P08104
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
