Overview
Type: Synthetic peptide
Form: Lyophilized powder
SCN4B/Navβ4 (extracellular) Blocking Peptide (#BLP-SC044) is the original antigen used for immunization during Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044) generation. The blocking peptide binds and ‘blocks’ Anti-SCN4B/Navβ4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates:1-4. Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044), (1:800).
5-8. Anti-SCN4B (NaVβ4) (extracellular) Antibody, preincubated with SCN4B/Navβ4 (extracellular) Blocking Peptide (#BLP-SC044).
Expression of NaVβ4 in rat DRGImmunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NaVβ4 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Properties
Sequence
- (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels (Accession Q7M730).
Accession (Uniprot) Number Q7M730
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
