Certificate of Analysis

Serotonin Receptor Antibody Explorer Kit

A Screening Package of Serotonin Receptor Antibodies Economically Priced
Cat #: AK-585 14 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-5HT1A Receptor (HTR1A) Antibody Cat #: ASR-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide KKSLNGQPGSGDWRRC, corresponding to amino acid residues 251-266 of rat 5-Hydroxytryptamine receptor 1A (Accession P19327). 3rd intracellular loop.
Homology
Mouse - identical.

Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody Cat #: ASR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B (Accession P28222). Extracellular, N-terminus.
Homology
Rat - 15/17 amino acid residues identical; mouse - 14/17 amino acid residues identical.

Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody Cat #: ASR-023

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)EASNRSLNATGAWD, corresponding to amino acid residues 14-27 of rat 5-hydroxytryptamine receptor 1D (Accession P28565). Extracellular, N-terminus.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody Cat #: ASR-033

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A (Accession P14842). Extracellular, N-terminus.
Homology
Mouse - identical; human - 9/13 amino acid residues identical.

Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody Cat #: ASR-035

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KMSEQ STISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B (Accession P30994). Extracellular, N-terminus.
Homology
Mouse - identical, human - 11/15 amino acid residues identical.

Anti-5HT4 Receptor (HTR4) (extracellular) Antibody Cat #: ASR-036

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4 (Accession Q62758). 2nd extracellular loop.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody Cat #: ASR-040

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide CSDSPSSGRSMG, corresponding to amino acid residues 22-33 of rat 5-Hydroxytryptamine receptor 5B (Accession P35365). Extracellular, N-terminus.
Homology
Mouse - identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.