sFTX-3.3

Synthetic Funnel Web Spider Toxin, Arginyl polyamine
A Blocker of P-Type CaV Channels
    Cat #: F-130
    Alternative Name Synthetic Funnel Web Spider Toxin, Arginyl polyamine
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 287.4
    Purity: >95%
    Effective concentration 0.1-1 mM.
      • sFTX-3.3
    Chemical name Pentanamide,2-amino-5-[(aminoiminomethyl)amino]-N-[3-[(3-aminopropyl)amino]propyl]-, (2S)-.
    Molecular formula C12H29N7O.
    CAS No.: 141997-14-0.
    Activity sFTX-3.3, a synthetic funnel web spider toxin1-2, is a polyamine amide analogue of FTX. It blocks P-, N-, and L-type Ca2+ channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies3.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
      • sFTX-3.3
        Alomone Labs sFTX-3.3 blocks P-type Ca2+ currents in Xenopus oocytes.
        A. Time course of P-type channel (CaV2.1+a2d1+b1a) activity before and during applications of 500 μM sFTX-3.3 (#F-130), and upon wash. Holding potential was -80 mV and currents were elicited every 10 seconds by 100 ms step to 0 mV. B. Superimposed current traces of P-type channels, before and during applications of 500 μM sFTX-3.3 (taken from the experiment described in A).
    References - Scientific background
    • 1. Cherksey, B.D. et al. (1991) Ann. N. Y. Acad Sci. 635, 80.
    • 2. Sutton, K.G. et al. (197) Comp. Biochem. Physiol. 116C, 23.
    • 3. Llinas, R. et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 85, 1689.
    • 4. Lin, J.W. et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 4538.
    • 5. Scott, R.H. et al. (1992) Br. J. Pharmacol. 106, 199.
    • 6. Blagbrough, I.S. et al. (1994) Tetra. Lett. 35, 2057.
    • 7. Moya, E. et al. (1994) Tetra. Lett. 35, 2061.
    • 8. Dupere, J.R.B. et al. (1996) Neuropharmacology 35, 1.
    • 9. Norris, T.M. et al. (1996) Mol. Pharmacol. 50, 939.
      • Polyamine amide spider toxins inhibit neuronal voltage-activated Ca2+ currents. A terminal arginine group is an important feature of these Ca2+ channel antagonists1-2.

        FTX is a polyamine component of the venom of the American funnel web spider Agelenopsis aperta, and has been described as a selective antagonist of P-type voltage-dependent Ca2+ channels3-4. However, it has been demonstrated that FTX has actions at other sites including NMDA and GABA receptors and also on T-type calcium channels5.

        sFTX-3.3, a synthetic funnel web spider toxin6-7, is a polyamine amide analogue of FTX. The structures of these polyamine containing compounds are not identical: sFTX-3.3 contains an amide carbonyl oxygen that is absent from the predicted structure of native FTX8. sFTX-3.3 has been shown to block P-type calcium channels in rat cerebellar Purkinje cells8 and antagonizes P-, N-, and L-type calcium channels in mammalian Purkinje and superior cervical ganglia neurons with similar potencies. Its IC50 against P-, N-, and L-type channels are ~0.24 mM, ~0.7 mM and ~0.7 mM, respectively9.

    Target P-type Ca2+ channels
    Last update: 03/03/2019

    sFTX-3.3 (#F-130) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use