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Certificate of Analysis

SK (Non-BKCa) Channel Antibody Explorer Kit

A Screening Package of SK (Non-BKCa) Channel Antibodies Economically Priced

Cat #: AK-218
Size: 13 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-KCNN1 (KCa2.1, SK1) Antibody Cat #: APC-039

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 (Accession P70606). Intracellular, N-terminal part.
Homology
Mouse - 17/19 amino acid residues identical; human - 12/19 amino acid residues identical.

KCNN1/KCa2.1 Blocking Peptide Cat #: BLP-PC039

Size: 40 µg

Anti-KCNN2 (KCa2.2, SK2) Antibody Cat #: APC-028

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542-559 of rat KCNN2 (Accession P70604). Intracellular, C-terminal part.
Homology
Mouse - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical; chicken - 14/18 amino acid residues identical.

KCNN2/KCa2.2 Blocking Peptide Cat #: BLP-PC028

Size: 40 µg

Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody Cat #: APC-028-GP (formerly AGP-045)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542- 559 of rat KCNN2 (Accession P70604). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical; chicken - 14/18 amino acid residues identical.

KCNN2/KCa2.2 Blocking Peptide Cat #: BLP-PC028

Size: 40 µg

Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody Cat #: APC-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCNN3 (Accession Q9UGI6). Intracellular, N-terminus.
Homology
Rat, mouse, pig - identical.

KCNN3/KCa2.3 (N-term) Blocking Peptide Cat #: BLP-PC025

Size: 40 µg

Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody Cat #: APC-103

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)KQIGSLESKLEHLTAS, corresponding to amino acid residues 659-674 of human KCNN3 (Accession Q9UGI6). Intracellular, C-terminal part.
Homology
Rat, mouse, pig - identical.

KCNN3/KCa2.3 (C-term) Blocking Peptide Cat #: BLP-PC103

Size: 40 µg

Anti-KCNN4 (KCa3.1, SK4) Antibody Cat #: APC-064

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4 (Accession Q9QYW1). Intracellular, C-terminal part.
Homology
Mouse, human, pig - identical.

KCNN4/KCa3.1 Blocking Peptide Cat #: BLP-PC064

Size: 40 µg

Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody Cat #: ALM-051

Size: 25 µg Host: Mouse Type: Monoclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Synthetic peptide mapping to the 3rd extracellular loop of human KCNN4 (Accession O15554).

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
A mouse monoclonal antibody (#ALM-051) and guinea pig polyclonal antibody (#APC-028-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
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