Overview
Type: Synthetic peptide
Form: Lyophilized powder
SLC17A9 Blocking Peptide (#BLP-NT085) is the original antigen used for immunization during Anti-SLC17A9 Antibody (#ANT-085) generation. The blocking peptide binds and ‘blocks’ Anti-SLC17A9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-SLC17A9 Antibody (#ANT-085), (1:200).
3,4. Anti-SLC17A9 Antibody, preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-SLC17A9 Antibody (#ANT-085), (1:400).
2. Anti-SLC17A9 Antibody, preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
Expression of VNUT (SLC17A9) in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC17A9 Antibody (#ANT-085), (1:2000), followed by goat anti-rabbit-AlexaFluor-488. A. VNUT immunoreactivity (green) in the paraventricular nucleus (PVN) of the hypothalamus is detected in neurons (vertical arrows) and axonal processes (horizontal arrows). 3V = third ventricle. B. VNUT staining (green) in the supra-optic nucleus (SON) of the hypothalamus is observed in neurons (vertical arrows) and axonal processes (horizontal arrows). OT = Optic tract. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SQKVQESERAFTYS, corresponding to amino acid residues 155-168 of mouse VNUT (Accession Q8VCL5).
Accession (Uniprot) Number Q8VCL5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
