SLC22A3 (extracellular) Blocking Peptide (#BLP-CT013) is the original antigen used for immunization during Anti-SLC22A3 (extracellular) Antibody (#ACT-013) generation. The blocking peptide binds and ‘blocks’ Anti-SLC22A3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Expression of OCT3 (SLC22A3) in mouse cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC22A3 (extracellular) Antibody (#ACT-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. OCT3 staining (green) in mouse cortex is detected in neurons (arrows). B. In mouse hippocampus, OCT3 immunoreactivity (green) is observed in astrocytes (vertical arrows) and neurons (horizontal arrows). Cell nuclei are stained with DAPI (blue).
- (C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83-96 of rat OCT3 (Accession O88446).