SLC41A2 (extracellular) Blocking Peptide (#BLP-NT146) is the original antigen used for immunization during Anti-SLC41A2 (extracellular) Antibody (#ANT-146) generation. The blocking peptide binds and ‘blocks’ Anti-SLC41A2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:
- Western blot analysis of human liver carcinoma HepG2 cell line lysate (lanes 1 and 2):
- Expression of SLC41A2 in mouse midbrain.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SLC41A2 (extracellular) Antibody (#ANT-146), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the substantia nigra region, showed SLC41A2 immunoreactivity (green) in the substantia nigra pars compacta (SNC). B. Pre-incubation of the antibody with SLC41A2 (extracellular) Blocking Peptide (BLP-NT146), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
- (C)KRSEPHLDYHEFSE, corresponding to amino acid residues 78-91 of mouse SLC41A2 (Accession Q8BYR8).