This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Alomone Labs Slotoxin inhibits mSlo channels heterologously expressed in Xenopus oocytes.Left: Time course of current amplitude changes upon application of 100 nM Slotoxin (#RTS-410), (bars represent the time of toxin perfusion). Right: An example of current response to 100 ms depolarization to +20 mV (from a holding potential of -100 mV) before (red) and during (black) perfusion of 100 nM Slotoxin.
Native Slotoxin was originally isolated from Centruroides noxius scorpion venom.
Native Slotoxin blocks the Maxi K+ (BKCa or slo, KCNMA1, KCa1.1) channels.1
Slotoxin blocks differentially channels formed by the α1 subunit alone and channels formed by the α1 combined with auxiliary β subunits. In Xenopus oocytes expressing hSlo (α1 alone), Kd was calculated to be 1.5 nM and the block is complete and reversible. With the additional co-expression of either the β1 or β4 subunits, the block become irreversible or causes the channel to be almost insensitive to the toxin, respectively.1
Slotoxin (#RTS-410) is a highly pure, recombinant, and biologically active peptide toxin.