Sortilin (extracellular) Blocking Peptide (#BLP-NT009) is the original antigen used for immunization during Anti-Sortilin (extracellular) Antibody (#ANT-009) generation. The blocking peptide binds and ‘blocks’ Anti-Sortilin (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate:
- Expression of Sortilin in rat neocortexImmunohistochemical staining of rat neocortex with Anti-Sortilin (extracellular) Antibody (#ANT-009). A. Sortilin appears in cortical neurons (red). B. Staining of interneurons with mouse anti-parvalbumin (PV, green). C. Confocal merge of Sortilin and PV demonstrates separate localization in neocortex.
- DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin (Accession Q99523).